Journal: Journal of Translational Medicine
Article Title: Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression
doi: 10.1186/s12967-025-06235-6
Figure Lengend Snippet: Activation of the Aerobic Glycolysis Pathway in EC. A Heatmap illustrating the enrichment scores of 84 canonical metabolism pathways in normal endometrium versus EC tissue. B Volcano plot of comparison of metabolism pathways GSVA scores between normal endometrium and EC tissue. C , D Box plot ( C ) and heatmap ( D ) demonstrate that the GSVA scores of the glycolysis pathway are consistently higher in EC compared to normal endometrium. E The GSEA plot reinforces the finding of aerobic glycolysis activation in EC. F A comparison of extracellular lactate concentrations among HEC-1B, Ishikawa cell lines, and patient endometrial glandular cells. G – I Bioinformatics analysis ( G ), RT-PCR ( H ), and immunohistochemical staining (Magnification: 200 ×) ( I ) consistently demonstrate upregulated expression of several key enzymes involved in the aerobic glycolysis pathway in EC. J Box plot of comparison of IHC scores of MCT4, LDHA, HK2, and GLUT3. K The Kaplan–Meier survival plot, based on the TCGA cohort, shows that high expression of HK2 and SLC16A1, predicts worse disease-free survival in EC. EC: endometrial cancer; GSVA: gene set variation analysis; GSEA: gene set enrichment analysis; TCGA: The Cancer Genome Atlas. *P < 0.05; **P < 0.01; ***P < 0.001; ns: no significance
Article Snippet: Endogenous peroxidase activity was neutralized, followed by incubation with specific primary antibodies against GLUT3 (1:5000, Cell Signaling Technology, #40538s), MCT4 (1:200, ABclonal, #A23384), LDHA (1:1000, Abcam, #ab47010), HK2 (1:500, Abcam, #ab227198), CD206 (1:1000, Invitrogen, #YD3888901A), N-cadherin (1:100, CST, #13116s), E-cadherin (1:1000, CST, #3195t), Vimentin (1:1000, Abcam, #ab8978), and CD31 (1:500, Beyotime, #AG1849).
Techniques: Activation Assay, Comparison, Reverse Transcription Polymerase Chain Reaction, Immunohistochemical staining, Staining, Expressing
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